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The epithelium consists of a single layer of cuboidal cells with a large number of microvilli on the apical surface. The basement membrane is a thin layer composed of a network of reticular fibers. Histochemically the basement membrane closely resembles that of the conjunctiva. The compact layer contributes to the tensile strength of the membrane. The fibroblast layer is the thickest layer of the AM made up of a loose fibroblast network. The outermost layer of the amnion is the spongy layer. The basement membrane is one of the thickest membranes found in human tissue. This layer is resistant to current cryopreservation techniques. The structural integrity, transparency and elasticity of the amniotic basement membrane make it currently the most widely accepted tissue replacement for ocular surface reconstruction. It is known to promote epithelial cell migration, adhesion and differentiation. It is an ideal substrate for supporting the growth of epithelial progenitor cells by prolonging their lifespan, maintaining their clonigenicity and preventing epithelial cell apoptosis. This action explains why AMT facilitates epithelialisation for PED with stromal ulceration. In tissue cultures AM supports epithelial cells grown from explant cultures and maintains their normal morphology and differentiation. The resultant cultured epithelium can be transplanted with the AM to reconstruct damaged corneas. The AM can be used to promote non-goblet cell differentiation of the conjunctival epithelium

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