Proteomics Research

Proteomics in Dental research includes: Identification of proteins associated with caries-causing bacteria Study of host response to caries Identification of biomarkers for early caries detection/ oral cancer biomarker Identification of proteins involved in enamel remineralisation Study of the role of saliva in caries development Identification of proteins involved in dental biofilm formation Identification of proteins involved in tooth erosion Identification of proteins involved in tooth wear Identification of proteins involved in tooth regeneration

Prestained Protein Marker (10-240 Kda),Three color bands(Green, Blue, Red). Ready-to-Use no need to boil.

Welcome to our proteomics services! We are dedicated to providing high-quality, reliable and cutting-edge proteomics solutions to meet the needs of our clients. Our team of experienced scientists and researchers are committed to delivering comprehensive proteomic analysis, including protein identification, quantification, post-translational modification analysis, protein-protein interaction studies and more. We utilize state-of-the-art mass spectroscopy and Bioinformatics tools to ensure accurate and precise results. Whether you are in academia or industry, we offer customizable proteomics services tailored to your specific research goals. From sample preparation to data analysis, we provide end-to-end support throughout the entire proteomics workflow. At our facility, we strive for excellence in customer service and scientific expertise. Our goal is to help advance your research by providing reliable data that meets the highest standards of quality and accuracy. Contact us today to learn more about how our proteomics services can benefit your research projects. We look forward to working with you! I wish to bring to your kind attention that we offer various Proteomics and Metabolomics services that are as follows: Protein Extraction and Purification Services: 1. Plant, Animal, Microbial Protein Extraction 2. 1D Gel Electrophoresis (SDS PAGE) 3. Iso electric focusing (On IPG Strip) 4. Protein Purification by Chromatography techniques 5. HPLC Reverse Phase Purity Check Protein Analytical Services: 1. MALDI Intact Mass, PMF, MALDI MS/MS, MALDI Data Analysis 2. QTOF Intact Mass, Protein Profiling, Peptide Mass Fingerprinting 3. QTOF N C -Terminal Sequencing Protein/ Peptide Sequencing 4. QTOF PTMs: Disulphide Bond Localization and Phosphorylation Analysis 5. QTOF Protein Label Free Quantification Protein Structural Analysis: 1. NMR: Proton NMR, Carbon NMR, 2D NMR 2. Circular Dichroism Scanning (Far UV/Near UV) 3. Intrinsic Fluorescence Spectroscopy 4. FTIR 5. DSC Other Proteomics Services: 1. UPLC Amino Acid Analysis 2. cIEF (pI Determination) 3. Isoform Separation by CZE Metabolomics Services: 1. Qualitative Quantitative Analysis of Fatty acids/Lipids/Carbohydrates: GCMS 2. Qualitative Quantitative Analysis of Fatty acids/Lipids/Carbohydrates: LCMS

Pefachrome C1E 5603 is a chromogenic substrate used to measure C1 Esterase Inhibitor (C1INH) activity in complement system studies. The C1INH is a serine protease inhibitor that regulates the complement cascade, playing a crucial role in the immune response.

Descriptions: Proteins control all biological systems in a cell, and while many proteins perform their functions independently, the vast majority of proteins interact with others for proper biological activity. Characterizing protein-protein interactions through methods such as co-immunoprecipitation (co-IP), pull-down assays, crosslinking, label transfer and far-Western blot analysis is critical to understand protein function and the biology of the cell.

​Operon Technologies offers specialized protein analysis services to accelerate your scientific exploration. Our state-of-the-art techniques empower researchers and industries to delve into protein identification, characterization, quantification, and more. With a focus on precision and innovation, Operon Technologies is your partner in unraveling the mysteries of proteins and driving breakthrough discoveries. Operon Technologies leads in proteomic services, unveiling hidden protein intricacies for pioneering discoveries. From identification to interactions, our advanced techniques empower researchers and industries to decipher proteins' roles. Partner with us to elevate your proteomic exploration and drive transformative insights. ​ Key Protein Services offered by Operon Technologies: ​ Protein Expression Expression in bacterial hosts Expression in Saccharomyces cerevisiae Expression in Pichia Pastoris Protein Purification Affinity tag-based purification Ion exchange-based purification Hydrophobic Interaction based purification Size exclusion chromatography Scale up to 1g of purified protein Protein Analysis Electrophoresis HPLC analysis Activity assay Polyacrylamide Gel Electrophoresis (PAGE) Native-PAGE SDS-PAGE ​ Iso-Electric Focusing (IEF) Separation on Polyacrylamide IEF gels (Invitrogen) Separation on Immobilized pH gradient (IPG) strips (Biorad/ GE Healthcare)​ ​ Two Dimensional Gel Electrophoresis (2 DE) Gel based separation of complex protein mixtures based on pI and molecular weight Expression analysis of protein spots using Image Master 2D Platinum-7 software (GE Healthcare) ​ High Performance Liquid Chromatography (HPLC) Purity Check Free and Bound Amino Acid analysis - Reverse Phase HPLC (RP-UPLC and Cation Exchange -HPLC) Size Exclusion HPLC Strong Cation Exchange HPLC Fast Protein Liquid Chromatography (FPLC) Purification of desired proteins Instrumentation ​ Circular Dichroism (CD) Spectroscopy Near and Far UV Spectra Secondary structure determination ​ Mass Spectrometry MALDI (Matrix Assisted Lazer Desorption and Ionization) LC-MS (Liquid Chromatography Mass Spectrometry) GC-MS ( Gas Chromatography Mass Spectrometry)

RapiZyme Proteinase K Digestion Module Achieve faster, more efficient nucleic acid analyses From R D to product manufacturing, gene therapies need to be comprehensively analyzed to ensure accurate, efficacious, and safe dosing. The nucleic acid content of a viral vectored gene therapy needs to be tested, and synthetic oligonucleotide therapies must be carefully studied for pharmacokinetic, pharmacodynamic, and metabolism information. Current assays cannot always effectively support sensitivity and accuracy requirements by regulators or leverage simple to implement sample preparation steps. Accessing the nucleic acid content from relevant sample and minimizing the interference of protein components is challenging, and the Waters RapiZyme Proteinase K Digestion Module is ideal for just that. The Waters RapiZyme Proteinase K Digestion Module is a ready to use, all-inclusive sample preparation kit containing three easy-to-use solutions for non-specific enzymatic degradation of proteins and improved workflow efficiency. The kit is certified to be free of exonucleases, endonucleases, and ribonucleases for high quality, accurate, and precise nucleic acid measurements. With the RapiZyme Proteinase K Digestion Module, your lab can easily prepare a protein-digested nucleic acid sample in as little as 40 minutes. Overview Perform non-specific enzymatic degradation of proteins for the efficient analysis of gene therapeutics in as little as 40 minutes with an easy-to-use, all-inclusive kit Facilitate the extraction of nucleic acid analytes from protein-containing samples when performing DMPK and ADME studies Disrupt protein binding that can interfere with the extraction of oligonucleotides from biofluids Release viral vectored genomes during the characterization of vectored gene therapies for seamless follow-up analyses

A serine protease that exhibits a very broad proteolytic activity. Hydrolyzes peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples.

Proteomics is a unique field of study of a specific proteome, including details on protein abundances, the variations and modifications, as well as their interacting partners and networks. It consisting of three main areas of research: Structural Proteomics, which investigates the 3D structure of a protein; Functional Proteomics, which focuses on protein protein(s) and protein other molecule(s) interactions; and Clinical Proteomics, dedicated to the study of protein as potential biomarkers of various diseases.

UltraPRO Reversible Stain for Western BlotsUltra FAST Silver Staining SystemUltra FAST Coomassie Stain UltraUTE Luminescent Substrate KitEasyDRY Gel Drying System and componentsZeroBINDING Staining Trays

Applications: Complete N-glycan removal for mass spec analysis Glycoprotein characterization (SDS-PAGE shift assays) Biopharmaceutical QC (antibody deglycosylation)

2D Gel Electrophoresis Services 1. Protein Quantification: By Lowery Method/ 2. Iso Electric Focusing (1-D Analysis): Tube Gel IEF IEF Strip.(pH 3-10 or specified otherwise on 7cm long tube or strip) 3. Protein Profiling by SDS-PAGE (2nd-D Analysis). We currently accept gel bands detected by Coomassie Blue R250, Silver stain or Fluorescent stains (i.e. Molecular Probes Sypro Ruby). We require Coomassie Blue R250 stained bands for identification of post-translational modifications and we strongly recommend that you use Coomassie Blue rather than silver (please see sample protocols) for sequencing. The facility is open to collaborative work with researchers in the development of novel methods and/or customizing existing methods for specific applications.

Proteinase K is a broad-spectrum serine protease used for the digestion of proteins and removal of contaminants during nucleic acid extraction from various biological samples.

Reliable and durable products designed for efficient performance in electrical and electronic applications. Made with quality materials, they provide consistent operation, easy installation, and long-lasting service. Suitable for a wide range of professional, industrial, commercial, and general-purpose applications.

Proteomics Services Our proteomic portfolio offers protein expression, purification and characterisation services, to fulfill your proteomics research requirements.Protein Expression Genei has several years of experience in protein expression in various host systems with or without various tags to suit researchers needs.Expression Systems which include:Yeast (Pichia pastoris and Saccharomyces cerevisiae).Bacteria (Escherichia coli, Pseudomonas and Bacillus).Insect Cell expression (Baculovirus).Mammalian Cells (CHO, HEK, Others).TagsHISMBPSUMOGSTFLAGProtein Purification: Our expertise is in purification of proteins using chromatography techniques including:Anion Exchange.Cation Exchange.Hydrophobic Interaction Chromatography (HIC).Metal Chelation Chromatography (Nickel, Cobalt etc.).Affinity Chromatography.Gel filtration Chromatography.Protein Characterization:Using a variety of techniques including :SDS PAGE.Western Blot.Protein MALDI-TOF and LC-MS/MS.Peptide Sequencing.N ¿¿¿ Terminal Sequencing.HPLC.ELISA.

Proteomics

Proteiomic Service

proteinase K is�a serine protease enzyme that is used to digest proteins, particularly in molecular biology applications.�It's known for its ability to degrade proteins, including those resistant to other proteases, and is often used to inactivate nucleases like RNases and DNases during DNA and RNA extraction.�

Type Or Paste Your Content Hereprotein Assays Protein Standard Protein Molecular Weight Marker Protein Transfer Buffer Protein Solubilisation Protein Controls Protein Enzymes Protein Extraction Protein Purification Dialysis System Protein Foldase Protein Detergents Zwitterionic Detergents Sds Detection Albumin Removal Protein Removal Phosphatase Assay Protease Assay Protease Substrate Protease Inhibitors Proteomic Grade Reagents Protein Buffers

Proteomics Facility is a state-of-the-art facility comprising of SYNAPT-HDMS equipped with nanoLC, ESI, and MALDI sources, two dimensional electrophoresis (2DE), gel documentation system, western blotting, fast protein liquid chromatography etc. The primary goal of this facility is to facilitate protein identification, quantification and characterization in various R D projects, for better understanding of the molecular mechanisms involved in biological processes. In the last few years, CSIR- NCL has made significant contributions to the area of mass spectrometry-based proteomics research.

In situ detection of DNA, RNA and proteins Our InsituPro, InsituPro VS and InsituPro VSi automate all steps of these labour-intensive methods. The InsituStain is our low-cost semi-automatic alternative.

Thermo Antibodies and tools for protein extraction, purification, modification and detection.

Proteomics Service

Proteomics Research

Proteomics

Proteomics Research Service

Proteomics Service

Proteomics